GE Microcal VP-ITC
Jul 26, 2018 1. Open Microcal Analysis Launcher and click on ITC200, which will launch Origin 7.0 (or open Origin 7 directly) 2. Import Data a. Click on Read Data b. Select data files to be added (include experimental titration and control titration) c. Click on Add files then OK 3. Review Raw Data The software will calculate baselines and the area of each peak. MicroCal PEAQ-ITC Control software update v1.30 Terms and conditions. I have read and agree to the terms of the Software licence.
Summary
The MicroCal VP-ITC (Isothermal Titration Calorimeter) unit directly measures heat evolved or absorbed in liquid samples as a result of mixing precise amounts of reactants. A spinning syringe is utilized for injecting and mixing of reactants. The user inputs the experimental parameters (temperature, number of injections, injection volumes) and the computer carries out the experiment. Origin® software is then used to analyze the ITC data using fitting models to calculate reaction stoichiometry (n), binding constant (KD), enthalpy (ΔH) and entropy (ΔS).
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To avoid damage or contamination, researchers must provide their own Hamilton 81414 syringe for loading sample solutions. The syringe can be purchased through VWR via CyBuy or directly from Hamilton. The instrument must be reserved in advance, and various rules apply. Reserve time using iLab. Reservations do not require approval.
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The instrument is located in 1238 Hach Hall. Enquiries should be directed to Sarah Cady. Phone 515-294-5975 (office). NOTE: The Microcal ITC is now serviced and supported by the Malvern company. The weblink is: http://www.malvern.com/en/products/measurement-type/microcalorimetry/default.aspx.
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Microcal Software
The instrument is to be used by students and other researchers. Significant training is required, including sucessful completion of several tutorials and lessons before you will be authorized to use the instrument. Staff will NOT provide methods development assistance for this instrument, but will direct you to internet-based ITC resources. The hourly rates are listed below. NOTE: There is a minimum charge of $10 for each use of the instrument.
User Category | Instrument Charge | Staff Assistance |
---|---|---|
ISU researchers | $2.70 | $30 |
Non-ISU academic researchers | $4.05 | $45 |
Non-academic users | $8.10 | $90 |
Microcal VPITC Resources
Microcal Origin Software
- Origin 7 Data Processing Lessons
- Lesson 1: Routine ITC Data Analysis and Fitting
- Lesson 2: Setting Baseline and Integration Range
- Lesson 3: Deleting Bad Data
- Lesson 4: Analyzing Multiple Runs and Subtracting Reference
- Lesson 5: ITC Data Handling
- Lesson 6: Modifying Templates
- Lesson 7: Advanced Curve Fitting
- Lesson 8: Autosampler Data (Optional Accessory)
- Lesson 9: Other Useful Details
- Appendix: Equations Used for Fitting ITC Data
- VPITC Users Manual (factory)